![]() An evaluation of two newly formulated calcium hydroxide cements: A leakage study. Jacobsen L, BeGole EA, Vitkus DD, Daniel DC. Moreover, seeking antimicrobial effects and stimulation of periapical tissues healing, root canal sealers based on calcium hydroxide became available in the late 1980s 5 5. Evaluation of cytotoxicity of two endodontic cements in a macrophage culture. Queiroz CES, Soares JA, Leonardo RT, Carlos IZ. ![]() 2020 39:703-720., although ZOE may induce periapical inflammation 4 4. Comprehensive review of current endodontic sealers. Komabayashi T, Colmenar D, Cvach N, Bhat A, Primus C, Imai Y. For decades, Endofill (Dentsply Maillefer, Providência, Santiago, Chile) and Pulp Canal Sealer (Kerr Sybron Endo, Orange, California, USA) prevailed in the Brazilian and USA market, respectively 3 3. Endodontic sealers based on zinc oxide and eugenol (ZOE) have been standard in endodontics since their development, based on their long-term success 3 3. Cytotoxicity Profile of Endodontic Sealers Provided by 3D Cell Culture Experimental Model. Silva EJNL, Carvalho NK, Ronconi CT, De-Deus G, Zuolo ML, Zaia AA. Hence, Endodontics has always required the use of materials well tolerated by apical tissues, presenting antimicrobial effects and promoting healing 2 2. ![]() Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials. Victoria-Escandell A, Ibañez-Cabellos JS, Cutunda SBS, Berenguer-Pascoal E, Beltrán-Garcia J, García-Lopez E et al. Although sealers are expected to be confined within the root canal space, sealer's contact with periapical cells by apical foramen may occur, interfering in periapical responses and resulting in delayed wound healing 1 1. The endodontic treatment culminates with the complete filling of root canal systems that avoid microbial infection. Sendo assim, MTA e Sealer 26 demonstraram compatibilidade biológica na presença de HDPSCs. MTA e Sealer 26 interferiram nas expressões de DMP1, OC / BGLAP e RUNX2 (p 0,05). Quando cultivado na presença de MTA e Sealer 26, as HDPSCs expressaram Nestin, CD105, NANOG e OCT-4 e não expressaram CD34 e CD45. Os dados foram analisados por ANOVA com correção de Bonferroni (p 0,05). O efeito na diferenciação celular foi determinado pela expressão dos genes RUNX2, ALP, OC/BGLAP e DMP1 por RT-PCR. A plasticidade celular foi avaliada pela determinação das expressões dos genes CD34, CD45, Nestin, CD105, Nanog e OCT4 por PCR. A viabilidade das HDPSCs foi avaliada pelos ensaios MTT e de exclusão de azul de tripano. Por esse motivo, a viabilidade celular e a expressão de genes envolvidos na plasticidade e diferenciação celular foram investigadas em células-tronco recuperadas de polpa dentária humana (HDPSCs) que estiveram em contato com quatro materiais endodônticos (Endofill, MTA, Pulp Canal Sealer e Sealer 26). Um material endodôntico deve ser minimamente prejudicial às células-tronco, uma vez que essas células são extremamente importantes, devido à sua capacidade de proliferação, autorrenovação e diferenciação celular. ![]() Mesenchymal stem cells cytotoxicity mineral trioxide aggregate genotoxicity dental pulp stem cells MTA and Sealer 26 showed biological compatibility in the presence of hDPSCs. MTA and Sealer 26 interfered with DMP1, OC/BGLAP and RUNX2 expressions (p 0.05). When cultivated in the presence of MTA and Sealer 26, hDPSCs expressed Nestin, CD105, NANOG, and OCT-4 and did not express CD34 and CD45. The data were analyzed using ANOVA with Bonferroni correction (p 0.05). The effect on cell differentiation was determined by RT-PCR expression of the RUNX2, ALP, OC/BGLAP, and DMP1 genes. PCR evaluated cellular plasticity by determining the CD34, CD45, Nestin, CD105, Nanog, and OCT4 expressions. ![]() The viability of HDPSCs was assessed by MTT and trypan blue exclusion assays. For this reason, in this in vitro study, the cell viability and the expression of genes involved in cell plasticity and differentiation were investigated in stem cells recovered from human dental pulp (hDPSCs) that were in contact with four endodontic materials (Endofill, MTA, Pulp Canal Sealer, and Sealer 26). An endodontic material must be minimally harmful to stem cells since they are essential, thanks to their capacity for cell proliferation, self-renewal, and differentiation. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |